The balance between fission and fusion regulates the morphology of mitochondria.
TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission
The deduced 152aa protein contains a central leucine zipper, a coiled-coil region, and a C-terminal transmembrane domain.
It also contains a region showing high interspecies homology and a putative tetratricopeptide repeat.
Immunocytochemical analysis of HeLa cells detected endogenous FIS1 expression in mitochondria.
Protease treatment indicated that FIS1 is expressed at the outer mitochondrial membrane.
Transfection of a C-terminal truncated FIS1 resulted in cytoplasmic expression, indicating that the C terminus is required for mitochondrial localization.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human FIS1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Asp121 of human FIS1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
