PCR analysis revealed expression in leukocytes, spleen, and brain as well as in fetal brain and most abundantly in a fetal skin library.
RT-PCR analysis also established that IL1HY1 expression is amplified in a stimulated macrophage cell line.
By EST database searching, Smith et al.
(2000) also identified a cDNA encoding IL1HY1, which they termed FIL1-delta.
Modeling indicated that IL1HY1 has a conserved 12-stranded beta-trefoil structure.
By searching EST databases for IL1-like ligands, Busfield et al.
(2000) also cloned IL1HY1, which they designated IL1RP3.
SDS-PAGE analysis indicated that IL1HY1 is expressed as a 23kD protein.
Northern blot analysis detected expression of a 2.8-kb transcript in placenta and uterus.
In situ hybridization analysis revealed constitutive expression by keratinocytes in vitro.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse FIL1d.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Asp156 of mouse FIL1d, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
