FGF basic is one of at least 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation.
Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway.
Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely.
The predicted 18kD FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted.
Transcription from alternate start sites produces 21-23kD forms found only in the nucleus.
High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH-3T3 cells.
The 18kD rat sequence has 98% aa identity with mouse, and 96-97% identity with human, bovine and sheep FGF basic.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse FGF2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Pro10-Ser154 of mouse FGF2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
