Fibulin-2 is an extracellular matrix protein, which belongs to the fibulin family.
This protein binds various extracellular ligands and calcium.
It may play a role during organ development, in particular, during the differentiation of heart, skeletal and neuronal structures.
Alternatively spliced transcript variants encoding different isoforms have been identified.
The protein predicted from the cDNA obtained from a mouse fibroblast library consisted of a 1,195-residue polypeptide preceded by a 26-residue signal peptide.
Except for 2 additional EGF-like repeats, the COOH-terminal region showed 43% sequence identity with fibulin-1.
The NH2-terminal 408 residues, unique to fibulin-2, showed no sequence homology to other known proteins and presumably formed 2 additional domains that differ in their cysteine content.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse FBLN2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Val896-Glu1106 of mouse FBLN2, fused to N-terminal His-Tag expressed in E. coli.
