Fas is a glycoprotein with a mass estimated at 43-48kD.
Fas is a member of the Tumor Necrosis Factor Receptor Superfamily (TNFRSF), and it shares a cytoplasmic motif with TNF RI, referred to as the death domain, that binds cytoplasm signaling molecules to trigger the cytoplasmic apoptotic signal .
Fas is expressed to a large extent on activated T and B lymphocytes, and on malignant lymphoid cells.
To a lesser extent, Fas is expressed on cells from liver, heart, kidney, ovaries, and on many other malignant cells.
Yan et al.
(2005) noted that the TNFRSF6 gene contains 9 exons.
They identified an antisense transcript SAF within the 12.1-kb intron 1 that is transcribed in the opposite direction as the TNFRSF6 gene.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat FAS.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Gln22-Leu171 of rat FAS, fused to a His-Tag expressed in 293F cell.
