By database searching, they determined that this protein is a member of a family of related proteins with 2 other members in mammals: FAM20B and FAM20C.
All FAM20 proteins contain putative conserved signal sequences as well as a conserved C-terminal domain.
Fam20a-GFP fusion proteins displayed perinuclear and cytoplasmic staining consistent with localization in the endoplasmic reticulum.
Transfection experiments in COS-1 cells demonstrated that Fam20a is a secreted glycoprotein, and immunofluorescence studies showed that an intact signal sequence is required for secretion.
Examination of the expression pattern of FAM20A from a panel of cDNAs derived from various human tissues revealed high levels in lung and liver and intermediate levels in thymus and ovary.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human FAM20A.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys368-Ser541 of human FAM20A, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
