FADS3 is a member of the fatty acid desaturase (FADS) gene family.
FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs.
FADS3 encodes a deduced 445aa protein that shares 52% and 62% sequence identity with FADS1 and FADS2, respectively.
All 3 proteins show significant homology to known desaturase enzymes, including 3 histidine motifs characteristic of membrane-bound desaturases, up to 4 transmembrane domains, and an N-terminal cytochrome b5-like domain, which is a common structural feature in a subset of desaturases from plants, animals, and yeast.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes human FADS3.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to Glu331-Gln445 of human FADS3, fused to His-Tag and T7-tag expressed in E. coli.