Fatty acid binding protein 5 was microsequenced, and degenerate oligonucleotides based on the amino acid sequence were used to screen a lambda-gt11 cDNA library prepared from noncultured psoriatic keratinocytes.
The PAFABP cDNA encodes a 135aa protein with molecular weight 15,164.
By Northern blot analysis, the authors detected elevated levels of PAFABP message in noncultured psoriatic keratinocytes as compared to normal keratinocytes.
PAFABP mRNA was also observed in transformed human epithelial cell lines, A431 epidermoid carcinoma cells, transformed human amnion (AMA) cells, and lymphoid Molt 4 cells.
PAFABP protein was increased in primary normal keratinocytes induced to differentiate, as determined by 2D gel electrophoresis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes porcine FABP5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Po
Immunogen：Recombinant protein corresponding to Met1-Glu132 of porcine FABP5, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
