The main role of factor VII (FVII) is to initiate the process of coagulation in conjunction with tissue factor (TF).
Tissue factor is found on the outside of blood vessels - normally not exposed to the bloodstream.
Upon vessel injury, tissue factor is exposed to the blood and circulating factor VII.
Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself.
The most important substrates for FVIIa-TF are Factor X and Factor IX.
The action of the factor is impeded by tissue factor pathway inhibitor (TFPI), which is released almost immediately after initiation of coagulation.
Factor VII is vitamin K dependent; it is produced in the liver.
Use of warfarin or similar anticoagulants impairs its function.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat F7.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ala315-Asp433 of rat F7, fused to His-Tag at N-terminal expressed in E. coli.
