Factor V is an essential cofactor of the blood coagulation cascade.
This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation.
This generates a heavy chain and a light chain which are held together by calcium ions.
The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin.
Factor 5 is a protein of the coagulation system, rarely referred to as proaccelerin or labile factor.
In contrast to most other coagulation factors, it is not enzymatically active but functions as a cofactor.
Deficiency leads to predisposition for hemorrhage, while some mutations (most notably factor V Leiden) predispose for thrombosis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse F5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gly1776-Pro2028 of mouse F5, fused to His-Tag at N-terminal expressed in E. coli.
