Factor XI is the zymogen form of factor XIa, one of the enzymes of the coagulation cascade.
Like many other coagulation factors, it is a serine protease.Factor XI (FXI) is produced by the liver and circulates as a homo-dimer in its inactive form.
The plasma half-life of FXI is approximately 52 hours.
The zymogen factor is activated into factor XIa by factor XIIa (FXIIa), thrombin, and it is also autocatalytic, and FXI is a member of the "contact pathway" due to activation by FXIIa (with includes HMWK, prekallikrein, factor XII, factor XI and factor IX).Factor XIa activates factor IX by selectively cleaving arg-ala and arg-val peptide bonds.
Factor IXa, in turn, activates factor X.Inhibitors of factor XIa include protein Z-dependent protease inhibitor (ZPI), which is independent of protein Z.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat F11.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ile275-Gly516 of rat F11, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
