As the only known physiological activator of thrombin, factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation.
Human factor X is initially synthesized in the liver as a single-chain precursor of 488aa residues with a signal peptide and a pro region (residues 1 - 40).
Both the intrinsic and extrinsic pathways activate factor X to Xa, which consists of light (residues 41 - 179) and heavy (residues 235 - 488) chains linked by a disulfide bond.
The light chain contains a Gla and two EGF-like domains and the heavy chain corresponds to the serine protease domain.
The full-length human factor X was expressed and the pro enzyme was purified and activated.
The active protease was further purified and analyzed for its activity towards either peptides or proteins containing a Xa cleavage site.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse F10.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gly21-Asn481 of mouse F10, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
