Prothrombin is a carbohydrate-protein compound in plasma essential to coagulation.
In response to bleeding, a complex series of clotting-factor interactions leads to its conversion by thromboplastin to thrombin, which transforms fibrinogen in plasma into fibrin.
Fibrin and platelets combine to form a clot.Prothrombin and prothrombin fragment F1+2 (F1+2) were demonstrated in the tumor stroma on cancer cells and on small blood vessels in areas of neoangiogenesis at the host-tumor interface.
F1+2 is an indicator of local activation of blood coagulation in cancer tissue.
Thrombin itself is impossible to quantitate and so the use of surrogate markers is necessary.
The measurement of F1+2 would be an excellent marker of thrombin generation.
This is helped by the fact that F1+2 is not generated in vivo by any other mechanism.
Fragment 1+2 has a half life of about 1 hour and is cleared from the bloodstream by the liver.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse F1+2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ala44-Lys324 of mouse F1+2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
