EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity.The deduced 747aa EZH1 protein has a calculated molecular mass of 85kD.
It shares about 55% aa identity (70% similarity) with the Drosophila protein.
EZH1 was expressed as 2 major transcripts of 5 and 6.5 kb in all adult and fetal human tissues surveyed; comparison of cloned cDNAs suggested that alternative splicing may account for at least part of the transcript size differences.
Analysis of an EZH1 cDNA revealed an unusual splicing event involving EZH1 and a tandemly linked gene, GPR2, suggesting a potential mechanism for modifying the EZH1 protein in the conserved C-terminal domain.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human EZH1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro559-Leu747 of human EZH1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
