(1996) described the cDNA sequence of envoplakin, a membrane-associated protein expressed in human stratified squamous epithelia in skin, oral mucosa, esophageal mucosa, and cervical mucosa.
They demonstrated that envoplakin is upregulated in the differentiated layers of the epidermis.
Envoplakin is homologous to the keratin-binding proteins desmoplakin I and II, bullous pemphigoid antigen-1 (BPAG1), and plectin.
Ruhrberg et al.
(1996) suggested that EVPL may be a novel desmosome component involved in anchoring keratin filaments to desmosomes in stratified epithelia.
They suggested that in human epidermis envoplakin may link desmosomes and keratin filaments to the cornified envelope.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human EVPL.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys30-Met258 of human EVPL, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
