Ethinyl estradiol (EE) is a derivative of 17beta-estradiol (E2), the major endogenous estrogen in humans.
EE is an orally bioactive estrogen used in many formulations of combined oral contraceptive pills.
It is one of the most commonly used medications for this purpose.
Transdermal ethinyl estradiol carries a greater risk of clot formation and venous thromboembolism than 17 beta estradiol, which some have theorized to be related to different amounts of hepatic metabolism after absorption.
The same contraindications and precautions apply for EE as with other estrogen medications.Estinyl was a preparation of EE alone that was used for the management of menopausal symptoms and female hypogonadism.EE is released into the environment as a xenoestrogen from the urine and feces of people who take it as a medication.
The major concern with unopposed estrogen is of endometrial cancer.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes Ethinylestradiol. Species Crossreactivity: pan-species (general)
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Immunogen：Small Molecule, Ethinylestradiol conjugated to OVA.
