Endoplasmic reticulum protein 29 (ERp29) is a protein which resides in the lumen of the endoplasmic reticulum (ER).
The protein shows sequence similarity to the protein disulfide isomerase family.
However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase.
The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
Alternative splicing results in multiple transcript variants encoding different isoforms.
Does not seem to be a disulfide isomerase.
Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes rat ERP29.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Rt
Immunogen:Recombinant protein corresponding to Asp42-Ser173 of rat ERP29, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.