SPFH2 associated rapidly with activated IP3 receptors , which are substrates for the ERAD pathway, in various mammalian cell lines.
A proportion of SPFH2 associated with several components of the ERAD pathway, and RNA interference-mediated depletion of SPFH2 inhibited polyubiquitination and degradation of IP3R1 and turnover of model ERAD substrates.
The deduced 339- and 152aa proteins share the first 141 N-terminal aa, then diverge.
Both proteins have an N-glycosylation site and type-2 membrane topology, and the longer protein has a lysine- and glutamic acid-rich region.
Northern blot analysis detected ubiquitous expression of 1.6- and 2.5-kb transcripts; a minor 4.4-kb transcript was also observed.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ERLIN2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro47-Asn339 of human ERLIN2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
