Epiregulin is a member of the epidermal growth factor family.
Toyoda et al.
(1995) purified a member of the epidermal growth factor (EGF) family, termed epiregulin.
Epiregulin functions as a tumor growth-inhibitory factor inducing morphologic changes in HeLa cells.
Purified mouse epiregulin contains 46aa residues with 24 to 50% sequence identity with other members of the EGF family and exhibits low affinity for the EGF receptor on human epidermoid carcinoma A431 cells.
Toyoda et al.
(1997) cloned and expressed the human epiregulin gene.
Their results showed that, although other members of the EGF family are ubiquitously expressed in normal tissues, the level of expression of epiregulin was extremely low but clearly detectable in macrophages and placenta and was high in carcinoma cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat EREG.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Val162 of rat EREG, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
