ErbB-4 is a member of the epidermal growth factor receptor subfamily.
ERBB4 is a single-pass type I transmembrane protein with multiple furin-like cysteine rich domains, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif.
The protein binds to and is activated by neuregulins-2 and -3, heparin-binding EGF-like growth factor and betacellulin.
Ligand binding induces a variety of cellular responses including mitogenesis and differentiation.
Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment.
Mutations in this gene have been associated with cancer.
Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ErbB4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Cys29-Asn278 of human ErbB4, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
