(1996) cloned and sequenced the human homolog of chick PG-Lb from 2 human chondrocyte cDNA libraries and a human chondrocyte RNA sample.
They named the human homolog dermatan sulfate proteoglycan 3 (DSPG3) and stated that it was the third member of the small dermatan sulfate proteoglycan family to be identified and characterized, with biglycan and decorin.
Deere et al.
(1996) mapped the DSPG3 gene to 12q21.
They confirmed the localization by PCR mapping of DSPG3 to a YAC contig map of chromosome 12.
The 1,515-bp DSPG3 gene encodes a predicted 322aa protein.
The protein contains 3 potential glycosaminoglycan attachment sites, 2 N-glycosylation sites, a polyglutamic acid stretch, and 6 cysteines.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse EPYC.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Cys130-Ile322 of mouse EPYC, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
