(2002) identified EPSTI1 as a transcript that is induced by coculture of breast tumor cells and fibroblasts, and they generated the full-length cDNA by 5-prime RACE of normal breast or placenta RNA.
The deduced 307aa protein has a calculated molecular mass of about 35kD.
It contains no N-terminal signal sequence and no transmembrane domains; however, there are 3 coiled-coil regions.
Northern blot analysis revealed a transcript size of 1.5 kb.
Real-time PCR revealed highest expression in placenta; strong expression in small intestine, spleen, salivary gland, and testes; and low levels of expression in normal breast and several other tissues.
The expression of EPSTI1 was upregulated up to 72 times (range 5.6-72.1) in all 14 breast carcinomas tested.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human EPSTI1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asn73-Glu280 of human EPSTI1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
