EPHX4 belongs to the AB hydrolase superfamily.In contrast to other members of the AB hydrolase superfamily, lacks the conserved Ser residue at position 169, which is replaced by an Asp residue, suggesting it has no hydrolase activity.
Epoxide hydrolase (also known as epoxide hydratase) functions in detoxication during drug metabolism.
It converts epoxides to trans-dihydrodiols, which can be conjugated and excreted from the body.
Epoxides result from the degradation of aromatic compounds.
Epoxides are significant as cytochrome P450 oxidase metabolites of unsaturated carbon-carbon bonds, but are also mutagenic.
Epoxide hydrolase is present in large quantity on endoplasmic reticulum.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse EPHX4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Lys36-Asp359 of mouse EPHX4, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
