The epithelial cell adhesion molecule (EpCAM, CD326) is a glycoprotein of ~40 kd that was originally identified as a marker for carcinoma, attributable to its high expression on rapidly proliferating tumors of epithelial origin.
Normal epithelia express EpCAM at a variable but generally lower level than carcinoma cells.
In early studies, EpCAM was proposed to be a cell-cell adhesion molecule.
However, recent insights revealed a more versatile role for EpCAM that is not limited only to cell adhesion but includes diverse processes such as signaling, cell migration, proliferation, and differentiation.
Cell surface expression of EpCAM may actually prevent cell-cell adhesion.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：chicken
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes chicken EPB42.
Isotype：IgY
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Ch
Immunogen：Recombinant protein corresponding to Asp21-Gly260 of chicken EPCAM, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
