Is a member of the G protein-coupled receptor family.
This protein is one of four receptors identified for prostaglandin E2 (PGE2).
Through a phosphatidylinositol-calcium second messenger system, Gq proteins mediate this receptor's activity.
Knockout studies in mice suggested a role of this receptor in mediating algesia and in regulation of blood pressure.
Studies in mice also suggested that this gene may mediate adrenocorticotropic hormone response to bacterial endotoxin.
Abundant in kidney.
Lower level expression in lung, skeletal muscle and spleen, lowest expression in testis and not detected in liver brain and heart.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (FFPE): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control (Supplied):362975A: Suppled as a recombinant protein EP1in 100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 0.02% BPB, 50mM DTT, 0.02% sodium azide, 50% glycerol
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human EP1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Val23-Pro184 of human EP1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
