Endothelial cells have the ability to regulate platelet activation, in part by the surface expression of ATP diphosphohydrolase (ATPDase; EC 3.6.1.5).
ATPDase hydrolyzes extracellular ATP and ADP to AMP, which is further converted to adenosine by another enzyme, 5-prime nucleotidase.
ADP is a powerful agonist for platelet recruitment and adhesion; adenosine is an antagonist of these processes.
CD39 was found to have both immunologic identity to, and functional characteristics of, vascular ATPDase.
By Northern blot analysis, Chadwick and Frischauf (1998) found that CD39 is expressed as a major 3.2- and a minor 3.6-kb mRNA in several tissues.
Additional bands were observed in a few tissues.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat ENTPD1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Leu213-Thr452 of rat ENPP2, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
