Endonuclease G, mitochondrial is a nuclear encoded endonuclease that is localized in the mitochondrion.
The encoded protein is widely distributed among animals and cleaves DNA at GC tracts.
This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
Tiranti et al.
(1995) mapped 3 human housekeeping genes involved in mitochondrial biogenesis.
One of these was the gene encoding a mitochondrion-specific endonuclease, designated ENDOG, that preferentially cleaves DNA stretches rich in C and G residues.
The authors hypothesized that this endonuclease could play a role in the maturation of RNA complementary stretches serving as primers for mtDNA replication, in the splicing process of polycistronic transcripts, or in mtDNA repair.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat ENDOG.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Lys294 of rat ENDOG, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
