ENA-78 is a CXC chemokine that was originally isolated from media conditioned by the growth of a mouse lung type-II alveolar epithelial cell line (A549) stimulated by IL-1 or TNF.
The full-length cDNA encodes a 114aa residue precursor protein with a 36aa residue signal peptide that is cleaved to generate the 78aa residue secreted protein.
ENA-78 shares significant aa sequence identity with NAP-2 (53%), GRO,, and (52%, 48% and 51%, respectively), and IL-8 (22%).
The gene for ENA-78 has been mapped to chromosome 4q13-q21.
Like other CXC chemokines, ENA-78 is a neutrophil attractant and activator in vitro.
Based on cross- desensitization experiments, it has been suggested that ENA-78 activity can be mediated through the IL-8 receptor system.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ENA78.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg45-Asn114 of human ENA78, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
