84-4415-57 EML2 (Echinoderm Microtubule Associated Protein Like Protein 2, EMAP2, ELP70, Echinoderm MT-Associated Protein (EMAP)-Like Protein 70) 200ul 362961
84-4415-57 EML2 (Echinoderm Microtubule Associated Protein Like Protein 2, EMAP2, ELP70, Echinoderm MT-Associated Protein (EMAP)-Like Protein 70) 200ul 362961
The nucleotide sequence of a human EMAP-related protein-2 (HuEMAP-2) encodes a protein of 649aa in length.
The translated polypeptide sequence and domain structure of sea urchin EMAP and HuEMAP-2 are highly conserved, with greater than 57% identity and 77% similarity at the translated aa level.
Southern blot analysis is consistent with the presence of a single HuEMAP-2 gene in the human genome.
Moreover, HuEMAP-2 is a member of a larger protein family with at least four HuEMAP sequences in the NCBI databases.
One of these, HuEMAP-1, is identified as the candidate gene for the Usher syndrome 1 a locus.
In addition, these RNA blots indicate that HuEMAP-2 transcripts may be differentially spliced in neuronal tissues.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes rat EML2.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Rt
Immunogen:Recombinant protein corresponding to Met1-Glu273 of rat EML2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.