EMILIN2 contains an N-terminal signal peptide with a consensus signal cleavage site, followed by an emilin (EMI) domain, several coiled-coil domains, a proline-rich region, a collagen-like coiled-coil alpha-helical region, and a C-terminal C1q domain.
The mature 1,023aa protein has a calculated molecular mass of 112kD.
EMILIN2 also has 8 potential N-glycosylation sites and 20 cysteines, a number of which are clustered as doublets that may form intramolecular disulfide bonds.
RNA dot blot analysis detected high EMILIN1 expression in fetal heart and adult lung.
Intermediate expression was detected in peripheral leukocytes, placenta, and spinal cord, and low expression was detected in fetal brain, spleen, thymus, and lung, and in adult heart, aorta, testis, bone marrow, small intestine, thymus, lymph node, and appendix.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry: 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes mouse EMILIN2. Species Cross Reactivity: rat.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa853-1074 of mouse EMILIN2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
