Elastase 4 is a member of the peptidase S1 family.
The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity.
Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.
These human caldecrin isoforms, like the rat preproenzyme, are composed of a signal peptide of 16aa, a propeptide of 13aa, and a mature form of 239aa.
Both recombinant caldecrins, produced in a baculovirus expression system, showed the same chymotrypsin-type protease activity and hypocalcemic activity.
The hypocalcemic activity of both caldecrin isoforms remained intact even after treatment with phenylmethylsulfonyl fluoride (PMSF) to abolish their protease activity.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ELA4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Val30-Leu268 of human ELA4, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
