Neutrophil elastaseis a serine protease in the same family as chymotrypsin and has broad substrate specificity.
Secreted by neutrophils during inflammation, it destroys bacteria and host tissue.
As with other serine proteases it contains a charge relay system composed of the catalytic triad of histidine, aspartate, and serine residues that are dispersed throughout the primary sequence of the polypeptide but that are brought together in the three dimension conformation of the folded protein.
The gene encoding neutrophil elastase, ELA2, consists of five exons.
The neutrophil form of elastase is 218aa long, with two asparagine-linked carbohydrate chains.
It is present in azurophil granules in the neutrophil cytoplasm.
There appear to be two forms of neutrophil elastase, termed IIa and IIb.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ELA2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ile30-Gln247 of human ELA2, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
