Elastase is an enzyme from the class of proteases (peptidases), that break down proteins.Elastase breaks down elastin, an elastic fibre that, together with collagen, determines the mechanical properties of connective tissue.
The neutrophil form breaks down the Outer membrane protein A (OmpA) of E.
coli and other Gram-negative bacteria, and also breaks down Shigella virulence factors.
This is accomplished through the cleavage of peptide bonds in the target proteins.
The specific peptide bonds cleaved are those on the carboxy side of small, hydrophobic aa such as glycine, alanine, and valine.
For more on how this is accomplished, see serine protease.Pancreatic elastase is a form of elastase formed in the pancreas.From recent research, it appears that of the two, ELA-1 is not transcribed into a protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ELA1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser40-Asn258 of human ELA1, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
