Early growth response protein 3 is a transcriptional regulator that belongs to the EGR family of C2H2-type zinc-finger proteins.
It is an immediate-early growth response gene which is induced by mitogenic stimulation.
The protein encoded by this gene participates in the transcriptional regulation of genes in controlling biological rhythm.
It may also plays a role in muscle development.
The human EGR3 gene was described by Patwardhan et al.
(1991) as predicting a 387aa protein containing 3 C2H2 zinc fingers nearly identical to those of EGR1 and EGR2.
The gene was known to be induced in various brain regions in response to stress or following focal brain injury.
in the SCN, it probably participates in the transcriptional regulation of genes in response to retinal input, as had been proposed for FOS.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human EGR3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly147-Cys403 of human EGR3, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
