The early growth response protein 2 is a transcription factor with three tandem C2H2-type zinc fingers.
Mutations in this gene are associated with the autosomal dominant Charcot-Marie-Tooth disease, type 1.
Using microarray expression profiling, Nagarajan et al.
identified 98 known genes that were induced by Egr2 in Schwann cells.
The putative Egr2 target genes included myelin proteins and enzymes required for synthesis of normal myelin lipids.
Using RT-PCR to monitor Schwann cell gene expression, Nagarajan et al.
confirmed that Egr2 is sufficient for induction of target genes including MPZ, PMP22, MBP, MAG, CX32, and periaxin.
Using EGR2 DNA-binding domain mutants, Nagarajan et al.
demonstrated a dominant-negative inhibition of wildtype Egr2-mediated induction of essential myelin genes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human EGR2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly147-Cys403 of human EGR2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
