Zif268 is a mammalian transcription factor that is now officially known as Egr1.It was originally discovered in mouse.
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins.
It is a nuclear protein and functions as a transcriptional regulator.
The products of target genes it activates are required for differentiation and mitogenesis.
Studies suggest this is a tumor suppressor gene.
The DNA binding domain of Zif268 consists of three zinc finger domains of the Cys2His2 type.
The aa structure of the Zif268 zinc finger domain is given in this table, using the single letter aa code.
The fingers 1 to 3 are indicated by f1 - f3.
The residues marked 'x' are not part of the zinc fingers, but rather serve to connect them all together.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human EGR1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln282-Ser433 of human EGR1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
