Diphtheria toxin and Pseudomonas exotoxin A (PA toxin) inhibit protein synthesis by catalyzing covalent binding of the ADP-ribose moiety of NAD to elongation factor-2 (EF2).
EF2 is required for the translocation step in protein synthesis, where peptidyl-tRNA is moved to the next codon on mRNA from the acceptor site on the ribosome at the expense of the energy provided by hydrolysis of GTP bound to EF2.
Class I diphtheria toxin resistance (sensitivity) is related to binding of the toxin, a function coded by chromosome 5.
Class II resistance is due to a defect in protein synthesis such that EF2 is not ADP-ribosylated by diphtheria toxin or PA toxin.
In one subclass this is due to a mutation in the structural gene for EF2; in a second subclass it is due to mutation in a gene for posttranslational modification of EF2.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human EEF2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys32-Val233 of human EEF2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
