EEA1 binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking.
It binds lipids through its FYVE domain and forms a homodimer through a coiled coil domain.
Sequence analysis predicted that the 1,411aa EEA1 protein is largely hydrophilic with short amphiphilic regions in the 15 N-terminal aa and in segments centered around aa 515 and 645.
EEA1 contains an N-terminal zinc finger motif, a cys-rich C-terminal metal-binding finger, and multiple sites for N-glycosylation, phosphorylation, N-myristoylation, and for a leucine zipper structure.
Immunofluorescence microscopy showed that EEA1 colocalizes with transferrin (TF) and with RAB5 (RAB5A), which localizes in early endosomes, but not with RAB7, which localizes in late endosomes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human EEA1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to His1232-Lys1348 of human EEA1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
