Endothelin 2 is a member of the endothelin protein family of secretory vasoconstrictive peptides.
The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events.
This gene product is involved in a wide range of biological processes, such as hypertension and ovulation.
The human endothelins represent a gene family comprised of endothelin-1, endothelin-2, and endothelin-3.
Based on the deduced amino acid sequences of the cloned ET2 and ET3 genes, corresponding proteins have been chemically synthesized and their vasoconstrictor activities studied.
Of the 3 isopeptides, ET-2 has the most potent vasoconstrictor activity.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human EDN2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln32-Arg178 of human EDN2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
