84-4415-07　ECE1 (Endothelin Converting Enzyme) 200ul　362911

特徴

• Endothelin converting enzyme 1, also known as ECE1, is a enzyme which in humans is encoded by the ECE1 gene.Endothelin-converting enzyme-1 is involved in the proteolytic processing of endothelin-1 (EDN1), -2 (EDN2), and -3 (EDN3) to biologically active peptides
• Schmidt et al.
• (1994) purified a membrane-bound protease activity from bovine endothelial cells that specifically converts the inactive form to EDN1.
• The enzyme was cleaved with trypsin and peptide sequencing analysis confirmed it to be a zinc-chelating metalloprotease containing the typical HEXXH (HELTH) motif.
• RT-PCR and cDNA screens were used to isolate the complete cDNAs of the bovine and human enzymes.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- HYRTSPF FSVYVSADSK NSNSNVIQVD QSGLGLPSRD YYLNKTENEK VLTGYLNYMV QLGKLLGGGD EDSIRPQMQQ ILDFETALAN ITIPQEKRRD EELIYHKVTA AELQTLAPAI NWLPFLNAIF YPVEINESEP IVVYDKEYLR QVSTLINSTD KCLLNNYMMW NLVRKTSSFL DQRFQDADEK FMEVMYGTKK TCLPRWKFCV SDTENNLGFA LGPMFVKATF AEDSKNIA
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.