Epstein-Barr virus induced gene 3 was identified by the induction of its expression in B lymphocytes by Epstein-Barr virus infection.
The protein encoded by this gene is a secreted glycoprotein, which is a member of the hematopoietin receptor family related to the p40 subunit of interleukin 12 (IL-12).
It plays a role in regulating cell-mediated immune responses.
EBI3 is a subunit in 2 distinct heterodimeric cytokines: interleukin-27 (IL27) and IL35
IL27 is composed of p28 (IL27) and EBI3.
IL27 can trigger signaling in T cells, B cells, and myeloid cells.
IL35, an inhibitory cytokine involved in regulatory T-cell function, is composed of EBI3 and the p35 subunit of IL12.Interleukin-27 (IL-27)is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (IL-27B) and IL27-p28 (known as IL-30).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse EBI3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Tyr19-Pro228 of mouse EBI3, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
