Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy.
Cytoplasmic dynein has been implicated in a variety of other forms of intracellular motility, including retrograde axonal transport, protein sorting between apical and basolateral surfaces, and redistribution of organelles like endosomes and lysosomes.
The predicted partial protein sequence shares 99% and 34% identity with rat DHC1a and human DNHC2, respectively.
Antibodies against DHC1 recognized a high molecular mass protein on Western blots of extracts from several mammalian cell lines.
Northern blot analysis revealed that DHC1 is expressed as an approximately 15-kb mRNA in several mammalian cells lines and human tissues, including those that make neither cilia nor flagella.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry nd Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry: 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol
Specificity：Recognizes human DYNC1H1
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala20-Leu285 from human DYNC1H1, expressed in E. coli
