Expression of DTX1 partially inhibited transactivation of a CSL (RBPSUH)-dependent luciferase reporter by activated intracellular NOTCH1 (ICN1) in human and mouse cells.
The N terminus of DTX1, which directly interacts with the NOTCH1 ankyrin repeats, inhibited transactivation by ICN1 possessing the ankyrin repeats, probably by inhibiting recruitment of coactivators to the C-terminal transactivation domain of NOTCH1.
The 620aa DTX1 protein contains an N-terminal domain predicted to associate with Notch ankyrin repeats, followed by a proline-rich region with potential SH3 domain-binding sites and a C-terminal domain that includes a classic RING-H2 finger motif.
DTX1 functioned as a ubiquitin E3 ligase.
It showed highest ubiquitination activity in conjunction with the E2 enzyme UBCH5A (UBE2D1).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DTX1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly290-His535 of human DTX1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
