The deduced 929aa protein contains a C-terminal kinase domain, which shares homology with the kinase domain of RIPK family members.
Northern blot analysis of human tissues detected weak expression in heart, brain, placenta, skeletal muscle, kidney, pancreas, and testis.
DSTYK failed to activate NF-kappa-B (NFKB1) in a reporter gene assay.
Overexpression of DSTYK induced DNA fragmentation and cell death in a dose-dependent manner with cell death occurring approximately 30 hours after transfection.
Caspase inhibitory protein and morphologic studies showed that DSTYK induced both caspase-dependent and caspase-independent cell death, and both the N- and C-terminal DSTYK deletion mutants retained the ability to induce cell death.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat DSTYK.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Pro650-Leu904 of rat DSTYK, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
