Destrin belongs to the actin-binding proteins ADF/cofilin family.
This family of proteins is responsible for enhancing the turnover rate of actin in vivo.
This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin).
Two transcript variants encoding distinct isoforms have been identified for this gene
In a variety of eukaryotes, destrin regulates actin in the cytoskeleton.
Destrin binds actin and is thought to connect it as gelsolin segment-1 does.
Furthermore, the binding of actin by destrin and cofilin is regulated negatively by phosphorylation.
Destrin can also sever actin filaments.
At a pH of 7.3 or higher it can sever the filaments but a lower pH the ability for it to do so is limited.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DSTN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Val165 of human DSTN, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
