Developmental pluripotency-associated protein 3 is a protein that in mice may function as a maternal factor during the preimplantation stage of development.
In mice, this gene may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality.
In humans, related intronless loci are located on chromosomes 14 and X
The deduced protein contains 150aa.
Northern blot analysis detected a 1.2-kb transcript expressed in male mouse gonads at 13.5 days postcoitum.
In situ hybridization of whole mouse embryos showed Dppa3 expressed initially in the gonads of both sexes, but expression was downregulated in female gonads by at least 13.5 days postcoitum.
Embryonic mice deficient in germ cells did not express Dppa3.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DPPA3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Pro159 of human DPPA3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
