Dipeptidyl-peptidase 2 is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes.
The resting lymphocytes are maintained through suppression of apoptosis, a state which is disrupted by inhibition of this novel serine protease.
The enzyme has strong sequence homology with prolylcarboxypeptidase and is active at both acidic and neutral pH.predicted 492aa protein has a calculated molecular mass of 54.3 kD and contains the consensus sequence for the active-site serine residue of serine-type proteases.
It shares 42%aa identity with the carboxypeptidase PRCP, with higher identity at the putative active-site residues.
Northern blot analysis detected a 1.7-kb transcript in PBMCs and Jurkat T cells.
SDS-PAGE analysis showed expression of a 58-kD protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DPP7.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp28-Gly486 of human DPP7, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
