DOK7 is essential for neuromuscular synaptogenesis.
The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes.
This protein can also induce autophosphorylation of muscle-specific receptor kinase.
Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B.
Alternative splicing results in multiple transcript variants.
DOK7 contains a pleckstrin homology (PH) and PTB domain in the N-terminal portion and Src homology 2 (SH2) domain target motifs in the C-terminal region.
Cloning of mouse and pufferfish Dok7 cDNA revealed a highly conserved structure.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat DOK7.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Trp63-Gly320 of rat DOK7, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
