The deduced 444aa mouse protein contains an N-terminal PH domain, a central PTB domain, and a proline-rich C-terminal domain.
Dokl is relatively serine/threonine-rich overall and contains several potential phosphorylation sites for ser/thr protein kinases.
Dokl shares 60% and 57% sequence similarity with p62dok (DOK1) over the PH and PTB domains, respectively, with lower similarity over the C-terminal regions.
Northern blot analysis of mouse tissues detected several species with high expression in spleen and lung and low levels in other tissues.
RNAse protection studies detected very high Dokl expression in bone marrow and spleen, low expression in thymus, and no expression in NIH3T3 cells.
Immunofluorescence studies localized Dokl to the cytoplasm in NIH3T3 cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat DOK3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ser45-Pro310 of rat DOK3, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
