DNMT3B, is a protein associated with immunodeficiency, centromere instability and facial anomalies syndrome.
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation.
Studies in mice have demonstrated that DNA methylation is required for mammalian development.
This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation.
The protein localizes primarily to the nucleus and its expression is developmentally regulated.
Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.
Six alternatively spliced transcript variants have been described.
The full length sequences of variants 4 and 5 have not been determined.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes mouse DNM3B
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ile581-Glu859 of mouse DNMT3B, expressed in E. coli
